Journal: Regenerative biomaterials

Article Title: Role of integrin β1 and tenascin C mediate TGF-SMAD2/3 signaling in chondrogenic differentiation of BMSCs induced by type I collagen hydrogel.

doi: 10.1093/rb/rbae017

Figure Lengend Snippet: Figure 5. (A) H&E staining. (B) Gene expression levels of ACAN, COL2A1 and SOX9. (C) Western blot detect the protein expression of COL2A1. (D) Semi- quantitation of gray level in (C). (E) Immunofluorescence staining for COL2A1. (F) Semi-quantitation of fluorescence intensity in (E) (scale bars: 170 lm. mean± SD, n ¼ 3; P 0.05, P 0.01, , ###P 0.001. represents the comparison between the experimental group and the control group, and # represents the comparison between the experimental groups).

Article Snippet: After blocking with normal goat serum, primary antibodies against TNC (1:100; Proteintech, Wuhan, China), ITGB1 (1:100; Proteintech, Wuhan, China), COL2A1 (1:100; Proteintech, Wuhan, China), COL1A1 (1:100; Proteintech, Wuhan, China), p-SMAD2/3 (1:100; Cell Signaling, USA) and SMAD2/3 (1:100; Cell Signaling, USA) were added and incubated overnight at 4�C.

Techniques: Staining, Gene Expression, Western Blot, Expressing, Quantitation Assay, Immunofluorescence, Fluorescence, Comparison, Control

Journal: Regenerative biomaterials

Article Title: Role of integrin β1 and tenascin C mediate TGF-SMAD2/3 signaling in chondrogenic differentiation of BMSCs induced by type I collagen hydrogel.

doi: 10.1093/rb/rbae017

Figure Lengend Snippet: Figure 8. Immunohistochemical staining for COL1A1 (A) and COL2A1 (B) in repaired cartilage after 4 and 8 weeks post-surgery (scale bars: 200 and100 lm).

Article Snippet: After blocking with normal goat serum, primary antibodies against TNC (1:100; Proteintech, Wuhan, China), ITGB1 (1:100; Proteintech, Wuhan, China), COL2A1 (1:100; Proteintech, Wuhan, China), COL1A1 (1:100; Proteintech, Wuhan, China), p-SMAD2/3 (1:100; Cell Signaling, USA) and SMAD2/3 (1:100; Cell Signaling, USA) were added and incubated overnight at 4�C.

Techniques: Immunohistochemical staining, Staining

Journal: Regenerative biomaterials

Article Title: Role of integrin β1 and tenascin C mediate TGF-SMAD2/3 signaling in chondrogenic differentiation of BMSCs induced by type I collagen hydrogel.

doi: 10.1093/rb/rbae017

Figure Lengend Snippet: Figure 9. Collagen hydrogel acts on ITGB1, followed by activating the ITGB1, then induces phosphorylation of SMAD2/3, the p-SMAD2/3 enters into the nucleus and binds to the promoter regions of TNC to initiate its transcription [58]. TNC increases the expression of ITGB1 and further induces activation of ITGB1 and SMAD2/3. p-SMAD2/3 promotes the stability and accumulation of SOX9 protein and forms a transcriptional complex with SOX9, promoting the expression of cartilage-related genes, like COL2A1, etc.

Article Snippet: After blocking with normal goat serum, primary antibodies against TNC (1:100; Proteintech, Wuhan, China), ITGB1 (1:100; Proteintech, Wuhan, China), COL2A1 (1:100; Proteintech, Wuhan, China), COL1A1 (1:100; Proteintech, Wuhan, China), p-SMAD2/3 (1:100; Cell Signaling, USA) and SMAD2/3 (1:100; Cell Signaling, USA) were added and incubated overnight at 4�C.

Techniques: Phospho-proteomics, Expressing, Activation Assay